Alix was born on February 2, 1576 at Remiremont in the Duchy of Lorraine to a noble Lorrainian family. She received a conventional aristocratic upbringing but underwent a personal-mystical conversion at age 21 (1597).
Alix sought the spiritual direction of St. Peter Fourier CanRegAug (1565–1640, Canon Regular of St. Augustine of the Congregation of Our Savior, see the existing entry for Pierre Fourier). Under Fourier's direction, Alix and four companions established the Congregation of Notre-Dame — the canonical form of Canonesses Regular of St. Augustine dedicated to the free education of girls (one of the earliest such congregations in the history of the Catholic Church).
The first Notre-Dame convent was established at Poussay in 1598 and moved to Nancy in 1603. Under Alix's direction, the Congregation of Notre-Dame expanded across Lorraine and eastern France with multiple daughter houses.
The pedagogical innovation of the Notre-Dame system — the free girls' school for both poor and rich together, with the curriculum including reading, writing, arithmetic, catechism, and domestic-and-artistic skills — established the Catholic girls' education tradition that became a model for the Catholic girls' education movement (St. Angela Merici's Ursulines had previously established the Ursuline tradition, but the Notre-Dame tradition was the canonical Congregation model).
Alix died at Nancy on January 9, 1622 at the age of 45.
Canonized January 22, 2014 by Pope Francis.